Robust Storage of Chinese Language in a Pool of Small Single-Stranded DNA Rings and Its Facile Reading-Out

Archival storage in DNA is one of the most challenging themes rapidly growing information technology. In addition, its practical applications are more difficult due to complicated data analysis, instability long and linear strands (>1000 nt), other factors. present study, we have developed a simple eminent DNA-based system which small rings employed as memory units. Compared with previous approaches, this methodology has advantages robustness, low cost, convenience so on. high density, Chinese language was directly stored pool 76-nt-long single-stranded (designated “Info-Store”), each ring memorized index number five characters (or marks). During “Read-Out”, all ssDNA were simultaneously amplified by hyper-branched rolling cycle amplification (HRCA), their sequences accurately determined portable MinION sequencer aided personal computer. Then, original text precisely smoothly decoded analysis. Books articles short rings, density. When it comes decipher, repeat obtained HRCA sequenced sequencer. Then these can be character This technology highly inclusive not limited Chinese.